Figure 1.

In vitro sensitivity of the Salmonella real-time polymerase chain reaction (qPCR) assay. Lymphocytes were isolated from 2–3 mL whole human blood by lysing red blood cells. Cell pellets were spiked with various concentrations of Salmonella Typhi Ty2, and DNA was isolated using a QIAamp Blood DNA Mini kit and tested using the clyA qPCR. The colored symbols represent 2 different users and the various symbols represent experiments performed on different occasions. Data are expressed as mean ± standard deviation from 3 replica wells. The maximum cycle threshold (Ct) value is 50 cycles. Wells with an undetermined Ct were assigned a Ct of 51.