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. 2014 Dec 23;105(3):353–360. doi: 10.1093/cvr/cvu260

Figure 1.

Figure 1

Expression of Tmem100 in developing organs. (A) Whole-mount X-gal-stained hearts of Tmem100+/SIBN mice at various embryonic, postnatal, and adult stages followed by clearing. X-gal-positive coronary arteries were indicated by arrowheads. LacZ expression was first detected at E17.5 (ii) and disappeared at weaning age (vi). Note X-gal-positive coronary artery (CA) and X-gal-negative coronary vein (CV) in the heart before clearing (iv). (B) Whole-mount X-gal-stained lungs followed by clearing (i–iv) and sections of X-gal-stained lungs counterstained with haematoxylin (v) or nuclear fast red (vi–vii). Only main pulmonary arteries were X-gal positive at E15.5 (i,v); however, LacZ expression spread as development proceeded (ii–iv and vi–vii). (C) X-gal-stained eye (i), spleen (ii), thymus (iii), and skin (iv) at P2 stage. Scale bars: A, Bi, and C, 500 μm; Bii–iv, 1 mm; Bv,vii, 50 μm; Bvi, 200 μm. More than three samples of each experiment were subjected to X-gal staining.