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. 2014 Jan 9;102(1):35–45. doi: 10.1093/cvr/cvu004

Figure 5.

Figure 5

DKK3 regulates ASK1 signalling in the heart. (A and B) The levels of phosphorylated and total MEK1/2, extracellular signal-regulated kinases 1/2, JNK1/2, and P38 proteins in samples from (A) WT and KO mice and (B) NTG and TG mice at the indicated times after a sham or AB operation (n = 3, *P < 0.05 vs. WT or NTG/sham; #P < 0.05 vs. WT or NTG/AB). (C and D) Levels of phosphorylated and total JNK1/2 and p38 proteins in samples of neonatal rat cardiomyocytes that were first infected with (C) AdshDKK3 or (D) AdDKK3 and then treated with Ang II for 60 min (n = 3, *P < 0.05 vs. AdshRNA or AdGFP/PBS; #P < 0.05 vs. AdshRNA or AdGFP/Ang II). (E and F) The levels of phosphorylated and total ASK1 proteins in samples from (E) WT and KO mice and (F) NTG and TG mice at the indicated times after a sham or AB operation (n = 3, *P < 0.05 vs. WT or NTG/sham; #P < 0.05 vs. WT or NTG/AB). (G and H) The levels of phosphorylated and total ASK1 proteins in samples of neonatal rat cardiomyocytes that were infected with (G) AdshDKK3 or (H) AdDKK3 and then treated with Ang II for 60 min (n = 3, *P < 0.05 vs. AdshRNA or AdGFP/PBS; #P < 0.05 vs. AdshRNA or AdGFP/Ang II). (top) Representative blots and (bottom) quantitative results. (I and J) ASK1 kinase activity in samples from (I) WT and KO mice and (J) NTG and TG mice at the indicated times after a sham or AB operation (n = 3, *P < 0.05 vs. WT or NTG/sham; #P < 0.05 vs. WT or NTG/AB). (K and L) ASK1 kinase activity in neonatal rat cardiomyocytes that have been infected with (K) AdshDKK3 or (L) AdDKK3 and treated with Ang II for 60 min (n = 3, *P < 0.05 vs. AdshRNA or AdGFP/PBS; #P < 0.05 vs. AdshRNA or AdGFP/Ang II). (left) Representative blots and (right) quantitative results. For this figure, n indicates the number of independent experiments.