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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Nat Protoc. 2018 Nov;13(11):2685–2713. doi: 10.1038/s41596-018-0058-x

Figure 4. Barcode copy number determination.

Figure 4.

Copy number of scGESTALT barcode integration was determined using qPCR. Samples 1–13 represent different genomic DNA samples (n = 13 animals). Reference standards: c1 is negative control (n = 1 animal), c2 is known to contain 1 copy (n = 1 animal), c3 is known to contain 2 copies (n = 1 animal). This procedure was approved by the HU/FAS Committee on the Use of Animals in Research & Teaching under Protocol No. 25–08.