| Component | Amount (μl) | Final Concentration |
|---|---|---|
| Mixture from Step 86 | 11 | |
| 5× PrimeScript buffer | 4 | 1× |
| Nuclease-free water | 3.5 | |
| RNaseOut (40 U/μl) | 1 | 2 U/μl |
| PrimeScript RT enzyme (200 U/μl) | 0.5 | 5 U/μl |
| Total | 20 |
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