| Component | Amount (μl) | Final Concentration |
|---|---|---|
| 5× Q5 reaction buffer | 5 | 1× |
| 10 mM dNTP | 0.5 | 0.2 mM |
| 10 μM inDrops_GP6 | 1.25 | 0.5 μM |
| 10 μM inDrops_PE1Sa | 1.25 | 0.5 μM |
| cDNA from Step 89 | 7.5 | |
| Q5 DNA polymerase | 0.25 | |
| Nuclease-free water | 4.25 | |
| Q5 enhancer | 5 | |
| Total | 25 |
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