Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT

. Author manuscript; available in PMC: 2019 May 1.

Published in final edited form as: Nat Protoc. 2018 Nov;13(11):2685–2713. doi: 10.1038/s41596-018-0058-x

Cycle number	Denature	Anneal	Extend	Final
1	98 °C, 30 s
2–16	98 °C, 10 s	61 °C, 25 s	72 °C, 30 s
17			72 °C, 2 min
18				8 °C, hold