| Component | Amount (μl) | Final Concentration |
|---|---|---|
| 5× HF Phusion reaction buffer | 5 | 1× |
| 10 mM dNTP | 0.5 | 0.2 mM |
| 10 μM inDrops_GP12 | 1.25 | 0.5 μM |
| 10 μM inDrops_PE1Sb | 1.25 | 0.5 μM |
| PCR product from Step 92 | 8 | |
| Phusion DNA polymerase | 0.25 | |
| Nuclease-free water | 8.75 | |
| Total | 25 |
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