| Component | Amount (μl) | Final Concentration |
|---|---|---|
| 2× NTP/CAP | 10 | 1× |
| 10× buffer | 2 | 1× |
| Linear, purified pCS2-zT2TP | 6 | ~20–30 ng/μl |
| Enzyme mix | 2 | |
| Total | 20 |
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