| Component | Amount (μl) | Final Concentration |
|---|---|---|
| Engen Cas9 NLS buffer | 1 | |
| Phenol red (0.1%, wt/vol) | 0.3 | 0.009% |
| sgRNA pool (~350 ng/μl each) | 1 | ~106 ng/μl |
| EnGen Cas9 NLS protein | 1 | |
| Total | 3.3 |
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