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. 2018 Sep 11;7(12):e1507600. doi: 10.1080/2162402X.2018.1507600

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — PRAME and HLA-I expression in synovial sarcoma and recognition by PRAME-T-cells.

a) PRAME expression in sarcoma as measured by mRNA-micro array. Horizontal line represents arbitrary cut-off value for PRAME positivity. Circles highlight high expression in all SS and EWS-NFATc2 translocation positive Ewing sarcomas. b-c) Primary SS (p ≤ 3) L2521 (b) and L2701 (c) were analysed by flowcytometry to assess total HLA-I surface expression after stimulation with 300u/ml of IFNα (IFNα) or 100u/ml IFNγ (IFNγ) for 18h. d) PRAME-T-cells (PRAME) were stimulated with primary SS cells L2521 and HLA-A2 transduced L2701 (L2701-A2), and SS cell line SYO1 transduced with HLA-A2 (SYO-1-A2). IFNγ production by the T-cells was measured after 18h of stimulation by standard ELISA. A CMV specific HLA-A2 restricted T-cell clone (CMV) served as negative control, and the USP11 specific HLA-A2 restricted T-cell clone (USP11) served as positive control. Synovial sarcoma cells were treated with 300u/ml of IFNα, (IFNα), 100u/ml IFNγ (IFNγ) or nothing (none) before stimulation.