Figure 6.

Trp shortage channels Trp towards WARS and protein synthesis.

(A) Cartoon depicting the mechanisms underlying the regulation of WARS by Trp-degrading enzymes. (B) WARS mRNA expression measured by qRT-PCR of LN18 glioblastoma cells treated with 0, 100 and 1000 U/ml of IFNγ in the presence and absence of 78 µM Trp. (C) Scheme depicting the most prominent flux changes in Trp metabolism upon Trp depletion. Fluxes were calculated by integration of microarray data from U87-MG glioblastoma cells into a computational model of Trp metabolism. Bar heights represent relative change in percentage between cells growing in normal media and cells growing without Trp. All data are expressed as mean ± s.e.m.