Table 1. Antibodies used for immunoblotting.
| Antibody | Host species | kD | Company | Cat. no. | Dilution |
|---|---|---|---|---|---|
| ERK1/2 | Rabbit | 42/44 | Cobb laboratory | Y691 | 1:3,000 |
| pERK1/2 | Mouse | 42/44 | Cell Signaling | 9106 | 1:2,000 |
| rpS6-p235/36 | Rabbit | 32 | Cell Signaling | 2211 | 1:1,000 |
| rpS6 | Mouse | 32 | Santa Cruz | sc-74459 | 1:3,000 |
| β-Catenin | Mouse | 92 | BD | 610154 | 1:1,000 |
| GSK3 α/β | Mouse | 51/46 | Santa Cruz | sc-7291 | 1:250 |
| pSer9/21-GSK3 | Rabbit | 51/46 | Cell Signaling | 9331 | 1:1,000 |
Secondary antibodies were donkey anti-mouse 800 and donkey anti-rabbit 700 from LI-COR Biosciences and were used at 1:10,000.