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. 2018 Dec 3;150(12):1606–1639. doi: 10.1085/jgp.201812203

Figure 4.

Figure 4.

Early recordings of voltage dependence of junctional conductance of pairs of Ambystoma blastomeres. Upper panels: Application of polarizing current (I1) to one cell (V1) of a coupled pair (V1, V2) causes the cells to uncouple (from Spray et al. [1979]). Middle panels: Voltage clamp of two coupled blastomeres. Voltage of one cell (V1) is kept constant, while the other cell (V2) is stepped to positive or negative voltages. The junctional current (Ij) is recorded as the clamp current applied to cell 1 (from Spray et al. [1981a]). Bottom panel: Plot of the steady-state junctional conductance–voltage relation of a pair of coupled blastomeres. Lines are Boltzmann fits to each polarity of voltage (from Spray et al. [1981a]). Fig. 4 top is reprinted with permission from Science.