Figure 2.

Recruitment of Asc1 requires the Rps3 tail. (A) Asc1 and the C-terminal tail of Rps3 are required for growth on glycerol, a nonfermentable carbon source. V.O., vector only. (B) Asc1 occupancy in ribosomes from WT or C-terminally truncated Rps3 (Rps3²¹⁴) cells was investigated by Coomassie-stained SDS-PAGE of purified 40S ribosomes. (C) In vitro binding of Asc1 to Asc1-deficient ribosomes containing full-length Rps3 or Rps3²¹⁴. Shown is the Coomassie-stained SDS–PAGE gel of the pellet fractions. The empty lanes on the right show that Asc1 alone does not pellet.