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. 2018 Dec 3;217(12):4141–4154. doi: 10.1083/jcb.201804163

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© 2018 Collins et al.

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Figure 3. — Ltv1 is required for stoichiometric assembly of Rps10 and Asc1. (A) The levels of Asc1 relative to Rps26 in ribosomes purified from WT or ΔLtv1 cells were determined by Western blotting. Data are averages from two technical repeats of three biological replicates (1–3). (B) Similar to ΔAsc1 cells, ΔLtv1 cells grow slowly in glycerol. Overexpression of Asc1 does not rescue this effect (n = 4). (C–E) Changes in the RP composition of the head structure were determined by Western blotting of ribosomes purified from WT and ΔLtv1 cells (C) or cells containing mutations in the Rps3 protein network (D and E). Note that the same gel is used and shown to evaluate Asc1, Rps10, and Rps26 levels in A and C. A separate gel is run to evaluate Rps8 relative to Rps10. The data are averages from four to six technical repeats from two biological replicates. In all cases, significance was determined using an unpaired t test, and the error bars represent the SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.