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. 2018 Dec 3;217(12):4230–4252. doi: 10.1083/jcb.201806161

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© 2018 Michaux et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 8. — Delayed accumulation of RGA-3/4 mediates negative feedback required for pulse termination. (A) Micrograph of a two-cell–stage embryo expressing GFP::RGA-3 (green) and NMY-2::RFP (red). (B) Temporal dynamics of a single pulse. (C) Top: Averaged normalized fluorescence intensities versus time for NMY-2::RFP and GFP::RGA-3 from two-color data, coaligned with respect to the time at which NMY-2::RFP reaches 25% threshold. The averaged normalized fluorescence intensity of GFP::AHPH, coaligned with NMY-2::RFP. Bottom: The averaged time derivative of the normalized GFP::AHPH intensity, again coaligned by using NMY-2::RFP. Data were averaged over 31 pulses in four embryos. Shaded regions represent 95% confidence intervals. (D) Top: Distributions of GFP::AHPH during interphase in zygotes with the indicated genotypes. Bottom: Kymographs showing patterns of GFP::AHPH distribution and redistribution during interphase for the same genotypes. Bars: (A and D) 5 µm; (B) 3 µm.