Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 3;217(12):4230–4252. doi: 10.1083/jcb.201806161

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Michaux et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 9. — Cortical RGA-3/4 localization depends on F-actin. (A) Micrographs of a zygote coexpressing GFP::RGA-3 and mCherry::LifeAct. (B) Magnified view of the region indicated by the dashed yellow box in A. (C) Scatter plots showing pixel-wise comparisons of GFP::RGA-3 and mCherry::LifeAct intensities for (left) the entire image shown in A or (right) for the duration of a pulse in the ROI shown in B. (D) Micrographs of a two-cell embryo coexpressing GFP::RGA-3 and mCherry::LifeAct. (E) Magnified view of the region indicated by the dashed yellow box in D. (F) Scatter plots showing pixel-wise comparisons of GFP::RGA-3 and mCherry::LifeAct intensities for (left) the entire image shown in E or (right) for the duration of a pulse in the ROI shown in E. (G) Wild-type zygote coexpressing GFP::AHPH and mRFP::LifeAct before (top) and ∼90 s after (bottom) treatment with 10 µM LatA. Middle panel shows a kymograph taken from the region indicated by the dashed yellow box in the top (pre-LA) micrograph. Blue rectangle indicates time of application of LatA. (H) Wild-type zygote coexpressing GFP::AHPH and mRFP::Myosin II before (top) and ∼90 s after (bottom) treatment with 10 µM LatA. Kymograph format as in G. (I) Homozygous unc-59(e261) mutant zygote coexpressing GFP::AHPH and NMY-2::RFP before (top) and ∼90 s after (bottom) treatment with 10 µM LatA. Kymograph format as in G. Bars: (A, D, and G–I) 5 µm; (B and E) 2 µm.