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. 2018 Dec 3;217(12):4314–4330. doi: 10.1083/jcb.201712130

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© 2018 Wang et al.

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Figure 5. — The loss of K6a/K6b proteins impairs the mechanical integrity of keratinocyte sheets in stationary culture. (A and C) Dispase-based assay revealed that Krt6a/Krt6b-null keratinocyte sheets display compromised stress resilience. (B) Quantification of the total number of fragments generated after 200 inversions. Data represent as the mean + SEM of four biological replicates. (D) Quantification of the total number of fragments generated after 100 inversions. Data represent the mean + SEM of two biological replicates. (E) By Western blot, the protein level of DP was reduced in the absence of K6a/K6b. Whole-cell protein lysates were harvested from keratinocytes that were grown in the same condition as that for the keratinocytes used in dispase-based assay. β-Actin was used as a loading control. n ≥ three biological repeats. (F) Data shown represent the mean + SEM of three biological replicates. *, P < 0.05; **, P < 0.01, Student’s two-tailed t test. M.W., molecular weight.