Figure 6.

Cells lacking all three Cdc42 GAPs retain pheromone-dependent polarization ability. (A) Percentage of cell pair formation of heterothallic h⁻ WT and rga3Δrga4Δrga6Δ cells (two independent strains) with h⁺ WT. n > 1,400 in three experiments. Error bars indicate SD. (B) Time-lapse images of Scd2-mCherry in h⁻ rga3Δrga4Δrga6Δ cells mating with unlabeled h⁺ WT. Two examples extracted from the same large group of cells illustrate dynamic polarization (top) and shmoo formation (bottom). (C) Medial spinning-disk confocal time-lapse CRIB-GFP images in h⁺ rga3Δrga4Δrga6Δ cells mating with unlabeled h⁻ WT. In B and C, the first image is a transmitted light image on which WT cells are marked with asterisks. Yellow arrowheads highlight assembly–disassembly patch dynamics. Green arrowheads indicate wandering patch movement. Orange arrowheads show the patch at the site of polarized growth. Red arrowheads point to fusion with partner cells. Time is in minutes from start of imaging. Bars, 3 µm.