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. 2018 Sep 25;7(12):e1466766. doi: 10.1080/2162402X.2018.1466766

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Both pre- and post-vaccine CD11 c+ cells can stimulate post vaccine CD8⁺ T cells to recognize UbiLT3 DRibble-derived tumor antigens. 4A. Dot plots from a pre/post crisscross experiment of one representative patient (out of 4 tested), looking at IFN-γ secretion by CD8⁺ T cells after the secondary stimulation with UbiLT3 DRibbles (top), NK DRibbles (middle) or plate-bound OKT3 (bottom) for the different combinations of pre- or post-DC with pre- or post T cells as indicated. 4B. Combined data of duplicate wells analyzed for two representative patients (out of 4 tested). The graph on the left corresponds with the patient data shown in 4A. 4C. Amounts of secreted IFN-γ in ng/ml. Data are shown for the 2 patients shown in B (out of 2 tested). 4D. TNF-α secretion data in pg/ml combined for the two patient samples depicted in 4B and C.