Figure 4.

For SB28, number of predicted MHC-I neoepitopes increases after in vivo growth. (A) Circos plot showing genomic distribution of mutations and predicted neoepitopes, representative example of in vitro SB28. From outer to inner, representation of mouse karyotype, point mutation (based on allele frequency (missense all freq), inner is 0 outer is 0.5), MHC-I predicted peptides (plotted according to affinity inner 13.4 nM, outer 33880 nM), MHC-II predicted peptides (plotted according to affinity inner 213.9 nM, outer 27834.1 nM), frameshift mutations. (B, C) numbers of expressed mutated genes and predicted neoepitopes for MHC-I and MHC-II binding.