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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Mol Cancer Res. 2018 Aug 21;16(12):1834–1843. doi: 10.1158/1541-7786.MCR-18-0289

Figure 1. USP6 induces an IFN response in ES cells in vitro and in primary tumors. A).

Figure 1.

Samples from primary Ewing sarcoma datasets (GSE7007 and GSE37371) were ranked by USP6 expression level, and GSEA was performed comparing the 5 samples with the highest levels to the 5 with the lowest. B) The indicated RD-ES cell lines were grown in the presence of doxycycline (dox) overnight, then blotted as indicated. The USP6 line represents a pooled population, whereas USP6(high) and USP6(Med) are clonal. C) RNA-sequencing was performed on USP6/RD-ES treated with or without dox, followed by GSEA pathway analysis. D) GSEA was performed for nodular fasciitis (NF) dataset 15, which utilized the Illumina Human HT12 v4.0 BeadChip. This platform contains one USP6 probe, which is specific for USP6. E) Relative USP6 levels were evaluated in samples from the germ cell tumor dataset GSE10615 (U133A Microarray). F) GSEA of the germ cell tumor dataset, comparing seminomas to yolk sac tumors.