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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Mol Cancer Res. 2018 Aug 21;16(12):1834–1843. doi: 10.1158/1541-7786.MCR-18-0289

Figure 4. IFNβ-Induced apoptosis requires Jak1-STAT1/STAT3 and entails extrinsic and intrinsic death pathways. A, B).

Figure 4.

Cells were treated with dox and IFNβ overnight, in the absence or presence of 50 μM pan-caspase inhibitor ZVAD (pan) or caspase-8 inhibitor IETD (8). Lysates were blotted as indicated. C, D) Cells treated with dox and IFNβ overnight, in the presence of caspase inhibitors as indicated, and caspase-9 (n=6) and caspase-3/7 activity was measured (n=3). Activity was calculated as fold relative to that in untreated USP6(High)/RD-ES. E) USP6(High)/RD-ES were treated with dox and IFNβ overnight, in the presence of a pan-Jak inhibitor (1 μM) or NFκB inhibitor PS-1145 (15 μM). F) Jak1, STAT1, or STAT3 were deleted by CRISPR gene editing. Cells were treated with dox and IFNβ overnight, then blotted as shown. Arrowhead indicates cleaved PARP product; ERK was used as a loading control.