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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Biomaterials. 2018 Oct 15;188:130–143. doi: 10.1016/j.biomaterials.2018.10.015

Fig. 4. The tumorigenesis of osteosarcoma cells and osteogenesis of osteoblasts cultured within scaffolds in vitro.

Fig. 4.

(A) HIF, VEGF, MMP2 and MMP9 mRNA expression in osteosarcoma cells cultured within scaffolds for 7 days. (B) Representative blots of HIFA, VEGF, MMP2, MMP9 and GAPDH in intrascaffold-cultured osteosarcoma cells after 7 days. (C) ALDH activity in intrascaffold-cultured osteosarcoma cells after 7 days. (D) The immunofluorescent staining of CD133 in intrascaffold-cultured osteosarcoma cells after 7 days. Scale bar: 10 µm. (E) ALP, BMP2, COL1 and RUNX2 mRNA expression in osteoblasts cultured within scaffolds for 7 days. (F) Representative blots of ALP, COL1, BMP2, RUNX2 and GAPDH in intrascaffold-cultured osteoblasts after 7 days. (G) ALP activity of intrascaffold-cultured osteoblasts after 7 days. (H) Alizarin red staining of intrascaffold-cultured osteoblasts after 7 days. Scale bar: 10 µm. n=3; mean ± SD; *P<0.05, **P<0.01, ***P<0.001; NS, not significant. L, M and H correspond to low (0.05% GelMA), middle (0.2% GelMA) and high (0.5% GelMA) adhesion ligand density.