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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Cancer Res. 2018 Oct 8;78(23):6539–6548. doi: 10.1158/0008-5472.CAN-18-0901

Figure 3.

Figure 3.

PTPN12, but not PTPN18, can trap tyrosyl phosphorylated ABL1. A, HEK293 T-REx cells, treated with tetracycline to induce expression of Flag vector (V), Flag-PTPN12-WT, or Flag-PTPN12-CSDA, were exposed to 50 μM PV or buffer control for 30 min before lysis. PTP-interacting proteins were co-immunoprecipitated with anti-Flag beads and identified by MS (combining results of both gel-free affinity purification and in-gel trypsin digestion). Heat maps show the relative amounts of each protein identified (compared with Flag vector control samples), as measured by label-free quantification. B, HeLa T-REx cells, treated with tetracycline to induced expression of Flag vector, Flag-PTPN12-WT, Flag-PTPN12-CSDA, Flag-PTPN18-WT, or Flag-PTPN18-CSDA, were exposed to 50 μM PV or buffer control for 30 min before lysis. Samples were immunoprecipitated with anti-Flag beads and immunoblotted with anti-ABL1, -pABL-Y245, -phosphotyrosine (pY) and -Flag Abs. ERK2 levels serve as a control for loading.