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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Gastroenterology. 2018 Aug 23;155(6):1883–1897.e10. doi: 10.1053/j.gastro.2018.08.025

Figure 6: Loss of MYO5B in adult mice leads to decreased apical expression of SI, AQP7 and DRA, but preservation of CFTR.

Figure 6:

(A) Immunostaining for p120 (green) and AQP7 (red) in control mice showed proper apical localization of AQP7. Tamoxifen-treated VillinCreERT2MYO5Bflox/flox mice lost apical expression of AQP7. (B) In control mice IAP (red) and SI (green) were both expressed on the apical membrane of of enterocytes. In VillinCreERT2MYO5Bflox/flox tamoxifen-treated mice SI was greatly reduced on the apical membrane and most SI appeared to be intracellular. IAP remained at the tips of the villi in VillinCreERT2MYO5Bflox/flox but also showed a subapical staining pattern through much of the villi (C) Immunostaining for DRA showed loss of apical DRA in tamoxifen-treated VillinCreERT2;MYO5Bflox/flox mice. (D) CFTR (red) and DPPIV (green) immunostaining in control mice demonstrated apical expression. In VillinCreERT2MYO5Bflox/flox CFTR was present below the apical membrane in the villi, however CFTR was also present on the apical membrane and appeared to be highly expressed in the crypts. n=4-6 mice per group, scale bars=50 μm.