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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Biol Cell. 2018 Sep 10;110(12):257–270. doi: 10.1111/boc.201800044

Figure 5. ARAP2-dependent changes in FAs are not dependent on Akt.

Figure 5.

U118 cells transfected with control or ARAP2 siRNA were plated on fibronectin coated plates or glass coverslips for 5 hrs and treated with 5μM Akt inhibitor MK2206 for 1 hour prior to lysis or fixation. (A) Akt inhibition by MK2206. Cell lysates were fractionated by SDS-PAGE and transferred to nitrocellulose for immunoblotting for the indicated proteins. (B) Effects of reduced ARAP2 expression and Akt inhibition on FAs. Fixed cells were immunostained for paxillin, imaged with immunofluorescence microscopy and FAs quantified. Results shown are the summary of three experiments, with quantification of FAs from 20-22 cells for each condition for each experiment.