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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Gastroenterology. 2018 Aug 23;155(6):1923–1935.e8. doi: 10.1053/j.gastro.2018.08.027

Figure 5: SREBP-2 mediates increased TM6SF2 expression during HCV infection.

Figure 5:

Huh7.5.1 cells were transfected with SREBF-1 siRNA, SREBF-2 siRNA or both for 72 h, and then infected with HCV. Total RNAs and proteins were obtained from those transfected cells at 96 h p.i. The relative expressions of TM6SF2 mRNA were determined in total RNAs after quantification of by qRTPCR and normalization with 18S RNA. Plotted data represent relative expression from 3 independent experiments in triplicates (n=9). Shown values are means ± SEM. NS, non-significant; **, P-value < .01; ***, P-value < .001 (Mann Whitney’s test). Proteins were detected by Western blot (Wes™ system), with the indicated antibodies.