Figure 2:

Genomic signature of the fecal microbiome associated with OCA administration. (A) A multidimensional scaling (MDS) plot of samples based on the Kendall rank correlation coefficient derived from 782 genes with a time-dependent effect in response to OCA administration based (repeated measure ANOVA, FDR<0.01). (B) Distribution of the 782 genes by bacterial taxonomy. (C) The abundance of a selected transposase (V8LYU6, from S. thermophilus) over time. (D) Out of 394 total transposases identified in the samples, 32 transposases had significant time-dependent responses to each of the three OCA doses (shown as log10 mean abundance and 95% confidence interval across samples). (E) ROC curves for transposases and plasma C4 (Red: transposase with highest AUC (0.917); Blue: transposase with lowest AUC (0.789); Black: plasma C4 levels (AUC = 0.828)).