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Catalytic parameters of cyclase activity in the presence of 5 and 10 μM of PHT in comparison with control (without PHT)
| Kinetic parameters | PHT = 0 μM | PHT = 5 μM | PHT = 10 μM |
|---|---|---|---|
| Vmax (nmol cGDPR/min/mg protein) | 160.13 ± 10.48 | 155.82 ± 11.27 | 153.45 ± 10.93 |
| Km (μM) | 150.95 ± 9.24 | 275.17 ± 13.72 | 420.35 ± 23.82 |
| α (inhibition efficiency) | 1 | 1.83 ± 0.03 | 2.8 ± 0.06 |
