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. 2018 Dec 4;8:17572. doi: 10.1038/s41598-018-35861-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Univariate reaction norms. Univariate responses to temperature (CGE1), temperature combined with food levels (CGE2) and temperature combined with loads of the insecticide Carbaryl (CGE3) in the three populations of D. magna resurrected from Lake Ring. Population reaction norms based on population means (n = 10) and SD, are shown for three life history traits. Responses in the life history traits to temperature, temperature combined with food levels and temperature combined with Carbaryl loads are shown. Only mortality data are available for the PP population at high concentrations of Carbaryl as the population experienced 100% mortality in this treatment three days after exposure (Fig. S1). The populations are colour coded as in Fig. 2.