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. 2018 Dec 4;8:17572. doi: 10.1038/s41598-018-35861-y

Table 1.

Analysis of variance.

CGE1 Fecundity Size at maturity Age at maturity Mortality
Chisq P-val Chisq P-val Chisq P-val Chisq P-val
Population (P) 1.422 0.491 0.074 0.964 1.753 0.416 3.765 0.152
Temperature (T) 8.563 <0.001 13.061 <0.001 5.102 0.024 2.043 0.153
P × T 3.494 0.174 1.908 0.385 1.162 0.559 4.121 0.127
CGE2
Population (P) 0.470 0.791 4.816 0.090 2.717 0.257 3.865 0.014
Temperature (T) 0.552 0.457 12.543 <0.001 100.929 <0.001 0.005 0.946
Food(F) 1033.209 <0.001 1202.270 <0.001 62.856 <0.001 0.415 0.519
P × T 0.034 0.983 0.178 0.915 4.343 0.114 8.472 0.014
P × F 0.102 0.951 1.322 0.516 1.419 0.492 0.283 0.868
T × F 0.001 0.978 1.707 0.191 6.604 0.010 3.494 0.062
P × T × F 0.153 0.926 0.140 0.932 0.768 0.681 3.365 0.186
CGE3
Population (P) 0.367 0.832 0.560 0.756 6.054 0.048 2.218 0.330
Temperature (T) 48.440 <0.001 67.951 <0.001 52.086 <0.001 11.494 <0.001
Insecticide (I) 13.619 <0.001 2.651 0.104 15.185 <0.001 82.991 <0.001
P × T 3.457 0.178 1.996 0.369 0.158 0.924 0.462 0.794
P × I 0.283 0.868 1.255 0.534 1.747 0.417 1.662 0.436
T × I 4.045 0.044 5.189 0.023 0.249 0.618 4.857 0.028
P × T × I 1.365 0.243 1.656 0.198 1.039 0.308 0.463 0.793

ANOVAs per single life history trait (mortality, fecundity, size and age at maturity) calculated for the three common garden experiments testing for the effect of population, treatment and their interaction terms. The populations are as in Fig. 1, each comprising 10 Daphnia magna genotypes and representing different time periods. CGE1 - Temperature treatment; CGE2 - Temperature and food levels; CGE3 - temperature and insecticide Carbaryl. Significant P-values are shown in bold.