Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 4;8:17611. doi: 10.1038/s41598-018-35464-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Identifying recombinant intrabodies using FLI-TRAP. (a) Schematic of engineered genetic selection for co-translocation of cognate intrabody-antigen pairs via the Tat translocase, TatABC. Here, an immune library of NAC-focused VHH genes was fused to a Tat signal peptide, namely spTorA, and co-expressed with a chimera between the antigen α-syn(A53T) and the reporter enzyme TEM1-Bla. By demanding cell growth on selective amounts of β-lactam antibiotics (e.g., Carb), intracellularly stable VHH clones that bind α-syn(A53T) can be readily isolated from the library. (b) Iterative workflow based on estimates from experiments for updating computational models and making new predictions.