Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 4;8:17608. doi: 10.1038/s41598-018-35710-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

BST-2 expression promotes proliferation-independent migration and invasion of breast cancer cells: (A) Giemsa-stained images of migrated MDA-MB-231 cells stably expressing scramble shRNA control (shCTL) and two different BST-2-targetting shRNA sequences (shBST-2h1 and shBST-2h2). (B) Quantification of migrated cells in panel A. (C) Microscopic images of Giemsa-stained 4T1 cells and (D) Graphs of 4T1 migration events in panel C. (E) Time-dependent trans-well migration of MDA-MB-231 cells. (F) Images showing kinetics of 4T1 wound closure. Black lines on images depict wound border (0 h) and extent of wound closure (12 h–24 h). (G) Quantitative depiction of wound closure events in panel F. (H,I) Images and quantitation of MDA-MB-231 invasion events. (J,K) Representative images and quantitation of 4T1 invasion. Representative images were taken at 4x magnification. Images used for Image J quantitation of migration and invasion were taken at 20x. Events from five different fields were analyzed. Error bars represent standard deviations. Significance was taken at P < 0.05* and P < 0.0001****. Experiments were repeated more than three times with similar results.