Fig. 1.

Kinome screen identifies GSK3 inhibitor that suppresses mutant KRas-dependent cells. a MiaPaCa2 (mutant KRas-dependent) and A549 (mutant KRas-independent) human tumor cells were treated for 72 h in 96-well plates with 304 kinase inhibitors (1 µM) using a one well-one inhibitor format. D [(% inhibition of viability of MiaPaCa2) − (% inhibition of viability of A549)] was determined for each compound based on the average of two screens. b Effects of SB on percent cell viability from both screens. c IC₅₀ determination of in-house synthesized SB (experiment done three times). d Western blots showing SB-induced caspase-3 activation and PARP cleavage in MiaPaCa2 but not A549 cells (experiment done three times)