Fig. 2.

Silencing of GSK3α/β induces apoptosis only in mutant (Mt) KRas-dependent cancer cells. Mutant KRas-dependent (MiaPaCa2, L3.6pl, SW620, and Calu-6) and mutant KRas-independent (A549, H460, DLD-1, and HCT-8) human cancer cell lines were a transiently transfected with SMARTpool KRas, GSK3α/β, or NT siRNAs or b treated with the GSK3α/β inhibitor SB and processed 72 h later for western blotting for KRas, GSK3α, GSK3β, c-caspase-3, c-PARP, and Vinculin. For a and b, experiments were repeated at least three times for each cell line