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Validation of RNA sequencing data. Quantitative real-time polymerase chain reaction using RNA collected from gastric cancer tissue (GC), huTGOs, cell lines derived from huTGOs (huTGOC), and AGS and NCI-N87 (NCI) cell lines for the expression of (A) GPD1, (B) CXCR4, (C) OLFM4, (D) IL13Rα2, (E) CA9, (F) KRT80, (G) AMIGO2, (H) CDKN2B, (I) KRT23, and (J) BAMBI. *P < .05 compared with GC samples, n = 3 assays per sample.
