Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 4;8:17458. doi: 10.1038/s41598-018-36143-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

EV interaction with PBMC. (a) The diagram of EV internalization in monocyte and lymphocyte PBMC fractions during 24 hours. Total PBMC fractions isolated from healthy donors were stimulated with EVs labeled with PKH67GL green fluorescent cell linker. After the indicated time periods cells were analyzed by FACS, and the statistics was performed on monocyte and lymphocyte gates separately (mean ± SEM, *p < 0.05 vs. cEVs, n = 5); (b) the representative images of FACS analysis of PBMC incubated with PKH67GL labeled EVs during 24 hours. The left diagram shows the lymphocyte fraction; the right diagram shows the monocyte fraction. (c) The diagram of PBMC proliferation rate after stimulation with EVs, analyzed by BrdU ELISA (mean ± SEM, *p < 0.01 vs. control PBMC, n = 3); (d) the surface marker expression on monocytes after EV treatment (mean % ± SEM, in bold - p < 0.05 vs. control PBMC, n = 4).