Table 1.
Gene | Primer | Product size (bp) | Annealing temperature (C) |
---|---|---|---|
GAPDH | F: AAGCTCATTTCCTGGTATGACAACG | 126 | 61.3 |
R: TCTTCCTCTTGTGCTCTTGCTGG | |||
PPAR-γ | F: ATGACAGACCTCAGACAGATTG | 210 | 54 |
R: AATGTTGGCAGTGGCTCAG | |||
LDLR | F: ACTTACGGACAGACAGACAG | 223 | 57 |
R: GGCCACACATCCCATGATTC | |||
IL-1 | F: GCTTCTCTCTGGTCCTTGG | 174 | 56 |
R: AGGGCAGGGTAGAGAAGAG | |||
IL-8 | F: GCAGAGGGTTGTGGAGAAGT | 150 | 56 |
R: ACCCTACAACAGACCCACAC | |||
TNF-α | F: GTCAACCTCCTCTCTGCCAT | 188 | 52 |
R: CCAAAGTAGACCTGCCCAGA | |||
TGF-β | F: TTGAGACTTTTCCGTTGCCG | 227 | 56 |
R: CGAGGTCTGGGGAAAAGTCT | |||
VEGF | F: CTTCTGAGTTGCCCAGGAGA | 216 | 54 |
R: CTCACACACACACAACCAGG |
GAPDH, glyceraldehyde-3-Phosphate dehydrogenase; IL-1, interleukin-1; IL-8, interleukin-8; LDLR, low-density lipoprotein receptor; PPAR-γ, peroxisome proliferator-activated receptor gamma; TNF-α, tumor necrosis factor alpha; TGF-β, transforming growth factor beta; VEGF, vascular endothelial growth factor.