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. 2018 Nov 28;9:2798. doi: 10.3389/fimmu.2018.02798

Figure 3.

Figure 3

Pre-stimulated T cells display extensive proliferation within RGD-PIC hydrogels, even at high cell densities. (A) Representative histograms of CFSE-labeled pre-activated pan T cells cultured in medium, collagen gels or RGD-PIC gels (1.5 mg/mL) for 3 days. Low cell density: 1 × 106 cells/mL, medium cell density: 2.5 × 106 cells/mL, high cell density: 5 × 106 cells/mL. (B–D) The percentage of proliferated T cells defined as cells that have undergone at least 1 proliferation cycle (B), the proliferation index defined as the number of cycles of proliferating T cells (C) and a quantification of the absolute number of cell retrieved (D) 72 h after transferring pre-activated T cells to medium, collagen or RGD-PIC gels at varying cell densities. Low cell density: 1 × 106 cells/mL, medium cell density: 2.5 × 106 cells/mL, high cell density: 5 × 106 cells/mL. (B–D) Values represent mean and SEM. Data were analyzed with the Kruskal Wallis test and the Dunns post-test. n = 2 or 3 in at least two independent experiments. *p ≤ 0.05.