Figure 1.

Sequencing data for MON810 35S promoter to show LAMP primer positions. (a) Positions of primer sequences on basic dumbbell amplicon produced after LAMP initiation. (b) New CaMV 35Sp sequence data from PCR amplified Mon810 transgene compared to GenBank EU760495.1, JX139718.1 and KJ608135 highlighting the partial nature of the 35S promoter sequence from JX139718.1. The alignments of the sequences are shown with the position of LAMP primers designed by Lee et al.²⁸ and used by Kiddle et al. (“K-primers”), highlighted as follows; displacement primers denoted F3 and B3 are in green, loop primers denoted by F-Loop and B-Loop are in yellow and the hairpin-forming LAMP primers FIP and BIP composed of the two sequences F2, F1c and B2, B1c as indicated and highlighted in red. Primers used by Zahradnik et al. 2014 (“Z-primers”) are also indicated with dashed lines.