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. 2018 Dec 4;8:17590. doi: 10.1038/s41598-018-36207-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LAMP amplification without the F3 displacement primer. BART detection using (a,b) Z-primers and (c,d) K-primers to show LAMP amplification with or without the F3 displacement primer. Individual reaction partitions s were run in wells of microtitre plates (see Methods). Partitions containing DNA template (n = 24) are in blue with no template controls (NTCs) (n = 24) in red. Traces show the measured light output in relative light units (RLU) over time for each individual reaction partition. The peak of light in the positive samples is due to LAMP amplification (see Methods). Maize genomic DNA (prepared from commercial Mon810 seed) at a calculated 208 copies 35S promoter per partition and standard primers subject only to desalting were used.