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. 2018 Dec 4;8:17590. doi: 10.1038/s41598-018-36207-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optimisation of the 35Sp K primer set. LAMP-BART time-to-peak (Tmax) results shown plotted against copy number of a linearised plasmid for; (a) comparison between K-primers in red and K+ primers in blue; (b) comparison between desalted primers in red and HPLC purified primers in blue. 8 replicates were run in each case. (c,g) Comparison between 0.5x concentration of K+ primers (0.8 micromolar LAMP, 0.4 micromolar Loop and 0.2 micromolar displacement primers) for 1 copy per partition using 96 replicates and LAMP-BART detection of linearised plasmid template with standard concentration of K+ primers described in Kiddle et al. (1.6 micromolar LAMP, 0.8 micromolar Loop and 0.4 micromolar displacement primers) (d,h) shows frequency histograms for Tmax distribution in (c,g). Comparison between native and denatured template with K+ primers: (e,f) LAMP-BART of 4 copies per partition native maize genomic DNA (Mon810), (i,j) LAMP-BART of 4 copies per partition denatured genomic template. 48 replicates were run in each case. (k) Amplification frequency for linear plasmid template using 1–104 copies; native in blue and denatured in red. (l) Predicted amplification frequency against copy number derived from Ucount^TM software for 0–5 copies.