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. 2018 Dec 4;8:17590. doi: 10.1038/s41598-018-36207-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LAMP-BART detection of 0.8 copies of 35S promoter or NOS terminator sequences in denatured maize genomic 0.1 percent Bt11 DNA extract. Column (A) K+ primer assay; column (B) Z primers; column (C) NOSt primer assay. The top row shows traces from replicate partitions of LAMP-BART assays for 0.8 copies per partition (n = 64) in blue and the NTCs (n = 8) in red. The frequency distribution of LAMP-BART Tmax time-to-peak results for each assay is below with a summary analysis table. HPLC grade primers and fast Bst v2.0 warm start polymerase were used for all three sets.