Table 3.

LAMP Primers.

Target, Type, Notation, Version	Length	Primer Sequence (5′ to 3′)
35Sp, Displacement, F3, DL	15	AGGAAGGGTCTTGCG
35Sp, Displacement, B3, DL	18	ATAAAGGAAAGGCCATCG
35Sp, LAMP, FIP, DL	39	GTCTTCAAAGCAAGTGG-TTTT-GGATAGTGGGATTGTGCG
35Sp, LAMP, BIP, DL	37	TTCCACGATGCTCCTCG-TTTT-CCTCTGCCGACAGTGG
35Sp, Loop, F-Loop, DL	16	TCCACTGACGTAAGGG
35Sp, Loop, B-Loop, DL	16	GGGGTCCATCTTTGGG
35Sp, Displacement, F3, CZ	18	AAGATGCCTCTGCCGACA
35Sp, Displacement, B3, CZ	19	CAGCGTGTCCTCTCCAAAT
35Sp, LAMP, FIP, CZ	39	ACGTGGTTGGAACGTCTTCTTCCCAAAGATGGACCCCCA
35Sp, LAMP, BIP, CZ	42	ATCTCCACTGACGTAAGGGATGATAGAGGAAGGGTCTTGCGA
35Sp, Loop, F-Loop, CZ	18	TCCACGATGCTCCTCGTG
35Sp, Loop, B-Loop, CZ	20	ACGCACAATCCCACTATCCT
NOSt, Displacement, F3, DL	22	CGCGATAATTTATCCTAGTTTG
NOSt, Displacement, B3, DL	19	CGTTCAAACATTTGGCAAT
NOSt, LAMP, FIP, PH	46	GCATGACGTTATTTATGAGA-TTTT-TCGCGCTATATTTTGTTTTCTA
NOSt, LAMP, BIP, PH	43	CATGCTTAACGTAATTCAACA-TTTT-TGAATCCTGTTGCCGGTC
NOSt, Loop, F-Loop, DL	22	GATTAGAGTCCCGCAATTATAC
NOSt, Loop, B-Loop, DL	23	AAATTATATGATAATCATCGCAA

Displacement, LAMP and loop primers which target the CaMV 35S promoter and NOS terminator sequences. Versions denoted with DL are primers designed by Lee et al.²⁸ and version CZ refer to primers described in Zahradnik et al.⁷. NOSt FIP and BIP LAMP primers were modified to improve assay performance. HPLC grade purified primers were used unless specified.