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. 2018 Nov 28;9:1777. doi: 10.3389/fpls.2018.01777

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Copyright © 2018 Zhong, Li, Hao, Fu, Ma, Xie, Shen, Kayani, Pan, Sun and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Expression analysis of AaABF3, ADS, CYP71AV1, DBR2, and ALDH1 in 3-month-old AaABF3-OE transgenic A. annua plants (A) and AaABF3-RNAi transgenic A. annua plants (B), respectively. (C,D) Contents of artemisinin in leaves of 3-month-old AaABF3-OE transgenic A. annua plants (C) and AaABF3-RNAi transgenic A. annua plants (D), respectively as determined by HPLC. (E,F) Expression analysis of AaABF3 (E) and ALDH1 (F) in AaABF3-RNAi transgenic A. annua plants at 0, 3, 9, and 12 h after ABA treatment. The β-actin was used as control in (A,B,E,F). Student’s t-test with P value determined whether the group difference was significant. ^∗P < 0.05; ^∗∗P < 0.01. Error bars represented the standard deviation (n = 3).