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. 2018 Nov 20;10:123–134. doi: 10.1016/j.isci.2018.11.027

Figure 2.

Figure 2

Vital Dye Method in Nematodes Allows Mixing Different Populations Together

(A) Neutral Red-stained adults (0.005% for 3 hours) imaged with Cy3 and FITC excitations and filters, and merged with DIC.

(B) An example of the relative intensities of fluorescence displayed as a histogram with the chemical structure of Neutral Red.

(C) CellTracker Green BODIPY (Thermo)-stained adults (50 µM for 3 hours) imaged with Cy3 and FITC excitations and filters, and merged with DIC.

(D) An example of the relative intensities of fluorescence displayed as a histogram with the chemical structure of CellTracker Green BODIPY.

(E) Combined worms from Neutral Red and CellTracker Green BODIPY staining on the same slide, merged with DIC.

(F) Age-dependent functional pheromone assay: experimental juveniles were stained with neutral red and challenged with CellTracker Green BODIPY-stained juveniles or adults on standard condition Nematode Growth Media (NGM) agar plates seeded with 300 μL OP50 E. coli. Three days later, only red-positive and green-negative adults were phenotyped.