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. 2018 Nov 20;10:123–134. doi: 10.1016/j.isci.2018.11.027

Figure 5.

Figure 5

Time-Resolved Nematode-Derived Modular Metabolites (NDMMs) in Pristionchus pacificus

(A and B) (A) Time-resolved secretion profile of nematode-derived modular metabolites from the wild-type laboratory strain RS2333 and (B) wild isolate RSC017. In both strains, at 24 hr cultures represent predominantly J2 stage worms, at 48 hr a mix of J2–J4, and at 72 hr predominantly adults in RSC017 (90%, Figure S4H) and a mix of J4/adults in RS2333 (Werner et al., 2017). Data are presented as the mean of 8 (RS2333) and 9 (RSC017) biological replicates, and error bars represent the standard error of mean (SEM).

(C–E) Chemical structures of age-specific NDMMs (C) dasc#1, (D) npar#1, and (E) ascr#9, as described in the Small Molecule Identifier Database (http://www.smid-db.org/), produced in ChemDraw.

(F–K) Time-resolved abundance of (F and G) dasc#1, (H and I) npar#1, and (J and K) ascr#9 NDMMs in RS2333 and RSC017. Each data point represents a biological replicate, and for comparison with (A and B) lines represent mean abundance. p values calculated by a 2-tailed Student's t test (***p < 0.001, **p <0.01, *p <0.05).