Fig. 7.

Decreased expression levels of mitochondrial respiratory chain complexes in H₂O₂-treated cochlear explants. (A) The organ of Corti explants of the Idh2^+/+ and Idh2^−/− mice were treated with H₂O₂ and/or MitoQ for 3 days. Altered expression levels of the oxidative phosphorylation (OXPHOS) subunits I, III and V were detected in the total protein lysates from H₂O₂-treated cochlea by Western blot analysis. β-actin served as a loading control. (B) Protein levels of OXPHOS complexes were calculated by densitometric analysis using ImageJ software (n = 3) Data are shown as the means ± SEM. *p < 0.05.